osteoblast-specific memo1 knock-out mouse model  (BioResource International Inc)

Journal: Physiological Reports

Article Title: FGFR regulator Memo1 is dispensable for FGF23 expression by osteoblasts during folic acid‐driven kidney injury

doi: 10.14814/phy2.15650

Figure Lengend Snippet: Primers used for qPCR.

Article Snippet: An osteoblast‐specific Memo1 knock‐out (Memo1 obKO) mouse model was established by crossing mice floxed for exon two of the Memo1 gene (Haenzi et al., ) backcrossed to the C57BL/6J background over at least 10 generations with Col1a1 Cre transgenic mice carrying a Cre recombinase under the control of the 2.3‐kb proximal fragment of the α1(I)‐collagen promoter (Dacquin et al., ; Haenzi et al., ) obtained through Riken BioResource Research Center (Ibaraki, Japan).

Techniques:

Journal: Physiological Reports

Article Title: FGFR regulator Memo1 is dispensable for FGF23 expression by osteoblasts during folic acid‐driven kidney injury

doi: 10.14814/phy2.15650

Figure Lengend Snippet: Validation of Memo obKO mouse model. Femoral Memo1 gene expression (a) and protein abundance (b) were blunted in Memo obKO, whereas Cre expression was detectable (c) in marrow‐free femur of Memo obKO. Memo1 expression was conserved in bone marrow (d), kidney (e) and liver (f) of Memo obKO animals relative to controls. Statistical analyses by Mann–Whitney U test. *, p < 0.05; ns, not significant. N of animals is indicated by the number of scatters. Animals were aged 9 weeks and both sexes at equal ratios in each group.

Article Snippet: An osteoblast‐specific Memo1 knock‐out (Memo1 obKO) mouse model was established by crossing mice floxed for exon two of the Memo1 gene (Haenzi et al., ) backcrossed to the C57BL/6J background over at least 10 generations with Col1a1 Cre transgenic mice carrying a Cre recombinase under the control of the 2.3‐kb proximal fragment of the α1(I)‐collagen promoter (Dacquin et al., ; Haenzi et al., ) obtained through Riken BioResource Research Center (Ibaraki, Japan).

Techniques: Biomarker Discovery, Gene Expression, Quantitative Proteomics, Expressing, MANN-WHITNEY

Journal: Physiological Reports

Article Title: FGFR regulator Memo1 is dispensable for FGF23 expression by osteoblasts during folic acid‐driven kidney injury

doi: 10.14814/phy2.15650

Figure Lengend Snippet: Three‐dimensional reconstructions of bone sites investigated by micro‐CT. Structure was not affected by loss of Memo1 in osteoblasts at any site, and 1 representative image per anatomical site and genotype is shown.

Article Snippet: An osteoblast‐specific Memo1 knock‐out (Memo1 obKO) mouse model was established by crossing mice floxed for exon two of the Memo1 gene (Haenzi et al., ) backcrossed to the C57BL/6J background over at least 10 generations with Col1a1 Cre transgenic mice carrying a Cre recombinase under the control of the 2.3‐kb proximal fragment of the α1(I)‐collagen promoter (Dacquin et al., ; Haenzi et al., ) obtained through Riken BioResource Research Center (Ibaraki, Japan).

Techniques: Micro-CT

Journal: Physiological Reports

Article Title: FGFR regulator Memo1 is dispensable for FGF23 expression by osteoblasts during folic acid‐driven kidney injury

doi: 10.14814/phy2.15650

Figure Lengend Snippet: Femoral μCT analysis of Memo1 obKO females ( n = 4).

Article Snippet: An osteoblast‐specific Memo1 knock‐out (Memo1 obKO) mouse model was established by crossing mice floxed for exon two of the Memo1 gene (Haenzi et al., ) backcrossed to the C57BL/6J background over at least 10 generations with Col1a1 Cre transgenic mice carrying a Cre recombinase under the control of the 2.3‐kb proximal fragment of the α1(I)‐collagen promoter (Dacquin et al., ; Haenzi et al., ) obtained through Riken BioResource Research Center (Ibaraki, Japan).

Techniques: Micro-CT, Control

Journal: Physiological Reports

Article Title: FGFR regulator Memo1 is dispensable for FGF23 expression by osteoblasts during folic acid‐driven kidney injury

doi: 10.14814/phy2.15650

Figure Lengend Snippet: Tibia μCT analysis of Memo1 obKO males ( n = 4).

Article Snippet: An osteoblast‐specific Memo1 knock‐out (Memo1 obKO) mouse model was established by crossing mice floxed for exon two of the Memo1 gene (Haenzi et al., ) backcrossed to the C57BL/6J background over at least 10 generations with Col1a1 Cre transgenic mice carrying a Cre recombinase under the control of the 2.3‐kb proximal fragment of the α1(I)‐collagen promoter (Dacquin et al., ; Haenzi et al., ) obtained through Riken BioResource Research Center (Ibaraki, Japan).

Techniques: Micro-CT, Control

Journal: Physiological Reports

Article Title: FGFR regulator Memo1 is dispensable for FGF23 expression by osteoblasts during folic acid‐driven kidney injury

doi: 10.14814/phy2.15650

Figure Lengend Snippet: Tibia μCT analysis of Memo1 obKO females ( n = 4).

Article Snippet: An osteoblast‐specific Memo1 knock‐out (Memo1 obKO) mouse model was established by crossing mice floxed for exon two of the Memo1 gene (Haenzi et al., ) backcrossed to the C57BL/6J background over at least 10 generations with Col1a1 Cre transgenic mice carrying a Cre recombinase under the control of the 2.3‐kb proximal fragment of the α1(I)‐collagen promoter (Dacquin et al., ; Haenzi et al., ) obtained through Riken BioResource Research Center (Ibaraki, Japan).

Techniques: Micro-CT, Control

Journal: Physiological Reports

Article Title: FGFR regulator Memo1 is dispensable for FGF23 expression by osteoblasts during folic acid‐driven kidney injury

doi: 10.14814/phy2.15650

Figure Lengend Snippet: Vertebral μCT analysis of Memo1 obKO males ( n = 4).

Article Snippet: An osteoblast‐specific Memo1 knock‐out (Memo1 obKO) mouse model was established by crossing mice floxed for exon two of the Memo1 gene (Haenzi et al., ) backcrossed to the C57BL/6J background over at least 10 generations with Col1a1 Cre transgenic mice carrying a Cre recombinase under the control of the 2.3‐kb proximal fragment of the α1(I)‐collagen promoter (Dacquin et al., ; Haenzi et al., ) obtained through Riken BioResource Research Center (Ibaraki, Japan).

Techniques: Micro-CT, Control

Journal: Physiological Reports

Article Title: FGFR regulator Memo1 is dispensable for FGF23 expression by osteoblasts during folic acid‐driven kidney injury

doi: 10.14814/phy2.15650

Figure Lengend Snippet: Vertebral μCT analysis of Memo1 obKO females ( n = 4).

Article Snippet: An osteoblast‐specific Memo1 knock‐out (Memo1 obKO) mouse model was established by crossing mice floxed for exon two of the Memo1 gene (Haenzi et al., ) backcrossed to the C57BL/6J background over at least 10 generations with Col1a1 Cre transgenic mice carrying a Cre recombinase under the control of the 2.3‐kb proximal fragment of the α1(I)‐collagen promoter (Dacquin et al., ; Haenzi et al., ) obtained through Riken BioResource Research Center (Ibaraki, Japan).

Techniques: Micro-CT, Control

Journal: Physiological Reports

Article Title: FGFR regulator Memo1 is dispensable for FGF23 expression by osteoblasts during folic acid‐driven kidney injury

doi: 10.14814/phy2.15650

Figure Lengend Snippet: Serum parameters of Memo1 obKO ( n = 4 per genotype and sex).

Article Snippet: An osteoblast‐specific Memo1 knock‐out (Memo1 obKO) mouse model was established by crossing mice floxed for exon two of the Memo1 gene (Haenzi et al., ) backcrossed to the C57BL/6J background over at least 10 generations with Col1a1 Cre transgenic mice carrying a Cre recombinase under the control of the 2.3‐kb proximal fragment of the α1(I)‐collagen promoter (Dacquin et al., ; Haenzi et al., ) obtained through Riken BioResource Research Center (Ibaraki, Japan).

Techniques: Control

Journal: Physiological Reports

Article Title: FGFR regulator Memo1 is dispensable for FGF23 expression by osteoblasts during folic acid‐driven kidney injury

doi: 10.14814/phy2.15650

Figure Lengend Snippet: Femoral μCT analysis of Memo1 obKO males ( n = 4).

Article Snippet: An osteoblast‐specific Memo1 knock‐out (Memo1 obKO) mouse model was established by crossing mice floxed for exon two of the Memo1 gene (Haenzi et al., ) backcrossed to the C57BL/6J background over at least 10 generations with Col1a1 Cre transgenic mice carrying a Cre recombinase under the control of the 2.3‐kb proximal fragment of the α1(I)‐collagen promoter (Dacquin et al., ; Haenzi et al., ) obtained through Riken BioResource Research Center (Ibaraki, Japan).

Techniques: Micro-CT, Control